Team:Waterloo/Notebook/Oligos

From 2008.igem.org

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* ' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene.
 
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* ' ' denotes that the construct contains the ribosome binding site, coding region for that gene, and a transcriptional terminator.
 

Revision as of 03:16, 30 October 2008

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Description Oligo Name Sequence Length
BioBrick sequencing/colPCR primer (fwd) iGEMvf2 TGCCACCTGACGTCTAAGAA 20
BioBrick sequencing/colPCR primer (rev) iGEMvr ATTACCGCCTTTGAGTGAGC 20
Primer for Lac promoter (fwd) PLacF TTATGCTTCCGGCTCGTATG 20
Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2) iGEMpre+rbs TTTCTCCTCTTTCTCTAGATGCGG 24
Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr) PrefixRBS-F GGCCGCTTCTAGAGAAAGAGG 21
Gene 5 ORF from T7 phage (fwd; Prefix adapter) T7gene5-F CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA 46
Gene 5 ORF from T7 phage (rev; Suffix adapter) T7gene5-R CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG 51
Gene 6 ORF from T7 phage (fwd; Prefix adapter) T7gene6-F CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA 46
Gene 6 ORF from T7 phage (rev; Suffix adapter) T7gene6-R CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG 43
PmeIR ORF (fwd) PmeIR-F ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG 50
PmeIR ORF (rev) PmelR-R CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG 52
pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAF AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA 71
pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAR CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG 71
{B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR) RBST7g6-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC 61
T7gene6 ORF (rev) T7gs-R CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC 48
T7gene6 ORF rev; for joining to PmeI with cross over PCR T7g6-RXO-RBS CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC 53
PmeI ORF (fwd; for adding prefix-RBS) RBSpmeI-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 61
PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) RBSpmeI-FXO TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 48
PmeI ORF (rev; TAATAA stop codon-suffix) pmeI-R CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG 45
Primer for part R0011 (Plac-PL hybrid promoter; fwd) R0011-F GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC 38
Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR) R0011RBS-RXO CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC 47
CI ORF (fwd; prefix-RBS) RBSC0051-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 59
CI ORF (fwd; prefix-RBS for joining to R0011 promoter by cross over PCR) RBSC0051-FXO TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 46
CI ORF (rev; suffix adapter) C0051-R CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG 40
CI ORF (rev; for joining to transcriptional terminator (b0015) by cross over PCR) C0051-RXO GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG 36
Transcriptional terminator (B0015; fwd; for joining to ORFs by cross over PCR) B0015-FXO TAATAATACTAGAGCCAGGCATCAAATAAAACG 33
Transcriptional terminator (B0015; rev) B0015-R CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC 39
RFP ORF (fwd; prefix-RBS) RFP-RBS-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG 58
RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by cross over PCR ) RFP-RXO GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG 34
Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR) Plac-RBS-F GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG 103