User:Jec105
From 2008.igem.org
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=== Summer Summary === | === Summer Summary === | ||
- | {{Imperial/Box2|| | + | {{Imperial/Box2|Our Approach|[[Image:Cycle.PNG|center|500px]]|}} |
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- | + | {{Imperial/Box1|Design| | |
+ | In order to achieve our specifications of design previously described, we require the following constructs; | ||
+ | *'''Light sensing device''' - Converting a light input into an output of PoPS, | ||
+ | *'''Biomaterial production device'''- Converting a PoPS input into an output of biomaterial, | ||
+ | *'''Motility Control device''' - Converting a PoPS input into an output of motility arrest, | ||
+ | *'''Integration device''' - To allow integration and selection of our genetic constructs, | ||
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+ | Here, AB is our antibiotic resistance cassette, ''ytvA'' is the gene controlling the light-sensing pathway, ''SB'' is the biomaterial, ''epsE'' the clutch and the 5' and 3' sections are integration sites. Light-inducible promoters are labelled with an 'L'. | ||
- | + | [[Image:Genetic circuit.PNG|750px|center]] | |
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- | {{Imperial/Box1| | + | {{Imperial/Box1|Modeling - Overview| |
Basically our dry lab team concentrated on characterising the chassis. In the dry lab section you'll find pages on the genetic circuit, growth curve and motility analysis from our project; this section will give a brief brief overview of each area (no pictures?). It will also include (in the motility analysis part) a movie (y/n?) of the motility response of ''B. subtilis'' with an inducible ''epsE'' gene BioBricked in. | Basically our dry lab team concentrated on characterising the chassis. In the dry lab section you'll find pages on the genetic circuit, growth curve and motility analysis from our project; this section will give a brief brief overview of each area (no pictures?). It will also include (in the motility analysis part) a movie (y/n?) of the motility response of ''B. subtilis'' with an inducible ''epsE'' gene BioBricked in. | ||
====== Growth Curve ====== | ====== Growth Curve ====== | ||
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- | {{Imperial/Box1| | + | {{Imperial/Box1|Implementation| |
- | + | Think what we can refer to implementation as 1)the construction of the parts ,2) the work with B.subtilis integration3)growth curve if we want to. Idea is to have three sub boxes with a large picture and brief text underneath. Last week Kirsten had the idea that we could make a big gel with the digests from all our parts, I will look into this week to get an image. \We could use this then put some stuff about no. of parts etc. Think}} | |
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- | + | {{Imperial/Box1|Testing| | |
- | + | Results from the B.subtilis motility and maybe the part characterisation | |
- | + | }} | |
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{{Imperial/Box1|Achievements| | {{Imperial/Box1|Achievements| |
Latest revision as of 08:16, 21 October 2008
Summer Summary
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