Alberta NINT/3 June 2008


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lab work (SD):

           Ran K102002/B+N and K102004/B+N on 2% agarose gel to purify.  
           Used QIAquick nucleotide removal standard protocol to purify T/A 0.  
           Ligated T/A 0 into K102002/B+N and K102004/B+N.

NINT SD p25.jpg

lab work (WM):

          #colonies K102011:    48           negative ctl:  0
          Picked 4 colonies for O/Ns