From 2008.igem.org
GROUP 1: Fluorescent Proteins
Date: July 1 2008
status report by: Ingrid (work done by James)
Part no.: BBa_K110017
Part Description: yESapphire
Part Location (in build a genome lab): In James and Jasper's PCR product Box,
Stainless Steel 4 degree
PCR successful?; Yes
Cloning of PCR product successful: Y/N
Sequencing of cloned PCR product successful: Not done
Joining of validated part to adjacent part(s) status: Not done
Current status of this part: This part is being Sequenced
Date: July 1 2008
status report by: Ingrid (work done by James)
Part no.: BBa_K110010
Part Description: yESapphire
Part Location (in build a genome lab): In James and Jasper's PCR product Box,
Stainless Steel 4 degree
PCR successful?; Yes
Cloning of PCR product successful: Y/N
Sequencing of cloned PCR product successful: No
Joining of validated part to adjacent part(s) status: Not done
Current status of this part: This part is being sequenced
Date: July 17 2008
status report by: Ingrid
Part no.: BBa_K110018
Part Description: mCherry:
Part Location (in build a genome lab): In silver fridge by door
PCR successful?; Yes
(http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471)
Cloning of PCR product successful: Inconclusive
Venus YFP and mCherry Miniprep check via digest
Sequencing of cloned PCR product successful: Not done
Joining of validated part to adjacent part(s) status: Not done
Problems to be solved: Had some extra unknown products, with unknown bands in the gel
Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
Date: July 17 2008
status report by: Ingrid
Part no.: BBa_K110019
Part Description: mCherry:
Part Location (in build a genome lab): In silver fridge by door
PCR successful?; Yes
(http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471)
Cloning of PCR product successful: Inconclusive
Venus YFP and mCherry Miniprep check via digest
Sequencing of cloned PCR product successful: No
Joining of validated part to adjacent part(s) status: Not done
Problems to be solved: Need to figure out if gel after digest shows correct products
Date: July 17 2008
status report by: Ingrid
Part no.: BBa_K110020
Part Description: Venus Enhanced YFP
Part Location (in build a genome lab): In silver fridge by door
PCR successful?; Yes
(http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471)
Cloning of PCR product successful: inconclusive
Venus YFP and mCherry Miniprep check via digest
Sequencing of cloned PCR product successful: No
Joining of validated part to adjacent part(s) status: Not done
Problems to be solved: Had some extra unknown products, with unknown bands in the gel
Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
Date: July 17 2008
status report by: Ingrid
Part no.: BBa_K110021
Part Description: Venus Enhanced YFP
Part Location (in build a genome lab): In silver fridge by door
PCR successful?; Yes
(http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471)
Cloning of PCR product successful: Inconclusive
Venus YFP and mCherry Miniprep check via digest
Sequencing of cloned PCR product successful: No
Joining of validated part to adjacent part(s) status: Not done
Problems to be solved: Had some extra unknown products, with unknown bands in the gel
Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
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