Illinois/10 June 2008

From 2008.igem.org

iGem Meeting Minutes 6/10/2008

The meeting began at 3:10

3:10 Aleem gave the presentation we made yesterday File:6-10-08.pdf

Advice from Professors (Rao and Golding):
- try expressing a T-cell receptor in yeast
- try using an FC receptor attached to an antibody-allows it to be generic and usable with various antibodies
- Potential Challenges:
  - Get yeast--ask Dr. Orleans. He is in India for a month but go to his lab. Another option is Dr. Zhao
  - We need a pheremone for the schmoos to come together
    - Can we buy a pheremone? we should be able to, but look into details.
  - We need a reporter
    - GFP or lacZ(colorimetric reaction). lacZ maybe a better choice.
- To Do this week and early next week:
  - Get yeast and begin culturing--it usually takes 2 days at room temperature in YPD media.
  - Get powdered media and pour it into dishes
  - Order NEB catalog
  - See what the yeast stock center in Seattle, has to offer
    - They should have free fluorescent proteins

4:00 Dr. Rao will take 4 people into his lab and teach them how to clone DNA

Dr.Rao explained the procedure for Tuesday's experiment
- Step 1 amplification-PCR
- Step 2 separation-gel electrophoresis
- Step 3 cut plasmid using restriction enzymes
- Step 4 add gene A by binding sticky ends with DNA ligase

4:40 We decided who was going to be in each group. The groups are posted on the Powerpoint. If you weren't at the meeting pick a group to work with and contact the group members.

There is additional info and pictures describing what Dr. Rao explained today at the end of the powerpoint.

The meeting ended at 5:00.