Making Overnight Cultures from Agar Colonies
GOLD MEDAL WINNER 2008
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- Pipette 10mL LB into red-capped culture tubes or conical shake flasks.
- Using an autoclaved toothpick, gently touch the point to a single colony.
- Drop the toothpick point down into the LB.
- Incubate overnight at 37°C whilst shaking.