Making Starch Agar Plates

From 2008.igem.org

  • Add 1g starch powder to 100mL LB.
  • Add 1g bacterial agar powder.
  • Mix by shaking gently and microwave until solute has completely dissolved. (Ensure lid of bottle is removed before microwaving.)
  • Pour into pre-labelled petri-dishes. 100mL LB should be enough to make 4 plates.
  • Remove any bubbles by quickly moving the flame of a Bunsen burner over the poured agar.
  • Leave to set 30-45 minutes.
  • Once set, leave the plates in a fan-cupboard for 10 minutes with their lids partially off to dry out any condensation before plating.