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Team:ESBS-Strasbourg/6 August 2008
From 2008.igem.org
Contents |
DryLab
Modeling
WetLab
Katja
- PCR confirms the identy of the Gal4O, LacIO, LexAO, cIO, tetO BioBricks (Which are in the pSB1A2 backbone and in the Silver-standard)
- Miniprep of the overnight cultures
- Digestion with SpeI and PstI of the LacIO, LexAO, cIO and the tetO vectors -> back vectors
- Ethanolic precipitation to get ride of the small fragments
Paul
-Digestion E+S of c1/lacI/lexA/tetR/EYFP/adh1-prom -> get insert
-Digestion X+P adh1-ter -> get insert
-Digestion E+X linker/Kozak -> get vector
-Digestion S+P NLS -> get vector
Assembly we want to perform:
c1+linker
lacI+linker
lexA+linker
tetR+linker
NLS+adh1term
EYFP+linker
adh1P+kozak
