Goals for the Week

  • Last week, had experiment with no current at all
    • Hypothesized that cells were dead?
    • Run test with 1 day vs. 2 day old Shewie to determine if there is an effect

Tuesday: July 22, 2008

  • started mtrB and wt cultures
    • mtrB culture to be used as 2-day culture on Thursday
  • troubleshoot LabVIEW
    • Resistances were measured to be different as expected in the past experiment
    • Some as low as 160 ohms
    • Perhaps the resistance of the chambers are less than we expected?

Wednesday: July 23, 2008

  • Started another mtrB culture
    • To be used as 1 day culture in experiment on Thursday
  • LabVIEW
    • Determined that resistance should be measured before connecting to the fuel cells
    • Resistance of chambers is the same as what we measured initially

Thursday: July 24, 2008

  • mtrB 1 day vs. 2 day experiment
    • 6 chambers
      • 3 chambers with 1-day old mtrB
      • 3 chambers with 2-day old mtrB
      • Injected lactate after the initial current spike came down
    • Hypothesis
      • 2-day cultures will have lower (if any) current production

Friday: July 25, 2008

Mtrb 1dv2d.jpg

mtrB: Overnight versus 2 Day Liquid Cultures

What we did:

-Ran 1-day old mtrB versus 2-day old mtrB


-The shapes of the curves were all very similar.

-Overnight cultures had slightly higher current production than 2-day old cultures.