GROUP 6: Vectors
Status report by Tejas on 8/5/08
Vector transformed into bacteria strain DB3.1: Yes. Pre-transformed by MIT and delivered in STABS
Permanent culture made in the Boeke lab for future reference: No
Selectable marker for this vector: (Amp, Kan, Cam)
Medium made and tested: No (link)
DNA preps made: Yes
DNA preps tested by RE digest: Yes, Vectors (CAM,AMP,KAN3,KAN4) after Digest
DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109): No (currently being done)
Sample format/data follows:
Amount transformed cfu/micGm in DB3.1 cfu/micGm in JM109
0.1 ng 5 * 10e7 <2 * 10e2
Preparative digests ready for use are located – where?
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