Team:NTU-Singapore/Notebook/18 July 2008

From 2008.igem.org

NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Friday 18 July

RESULTS FROM THURSDAY TRANSFORMATION: Plate of E7 got 4 colonies!! Plate of T7ptag got 8 colonies!! We also received LsrA and Lysis (lyophilized form) in Bluescript vector. We eluted them using TE buffer. Afternoon: 1600: transform lysis and LsrA (0.3ul each) into competent cells. 1700: inoculate 4 colonies of E7 and 8 of T7ptag.

Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/18_July_2008"

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers