Personnel

Hung(Greenbear), Zhen Fu

Experiment

• • Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
    1. 1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
    2. 1 LB plate, half for BW, half for W3110
  • Amplification of LacI-GFP (BBa_J04430) and Terminator (BBa_B0015) parts.
  • Overnight incubation at 37 degrees C of these 3 agar plates:
    1. LB AmpR spread with 250ul cells with LacI-GFP plasmid.
    2. LB AmpR spread with 125ul cells with Terminator plasmid.
    3. LB KanR spread with 125ul cells with Terminator plasmid.
  • Inoculation of bacteria containing Colicin E7 plasmid (without AmpR) received from Taiwan. We inoculated the cells in 4 cryotubes, which were then put into 37 degrees incubator for overnight incubation:
    1. 2 tubes each containing 5ml LB broth with Ampicilin. (which we expect not to see any bacteria in the next morning)
    2. 2 tubes each containing 5ml LB broth without Ampicilin.