Team:The University of Alberta/21 July 2008

Transformed the ligation of Tryp into J6
Finished Westerns of the Butanol suff in J6...RESULTS PENDING
- Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason.
Worked out the procedure for putting together all of our parts.
Re-digesting Lac1 ERE, TetR. RBS.6x His, RBS-6xHis, TDNA-MCS with Xba/Pst.
Digested BisDA and BisDB with Xba/Pst. Ran them on gel.
- They looked good, so gel purified the bands.
Cut I0500 with Spe and Pst. No bands appeared on gel. Reran them on gel and got two bands.
- Set up the O/N's using the colonies on plate. Minipreps to be done on Tuesday.