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Team:University of Ottawa/23 July 2008
From 2008.igem.org
Contents |
Today in the lab
Dan
- PCR amplification of T123
- I wanted to do PCR amplification of 0A and 0B, however PCR blocks were full, I will do this tomorrow
- 6 tubes of PCR reaction were prepared for T123, hopefuly this will give me the amount of DNA that I need for a successful ligation.
- Transformation of p2S and p2D
- p2S and p2D were transformed in E. coli
Chris
- Minipreparation of AtCRE
- Used minprep kit and protocol to isolate AtCRE from inoculated cells.
- Measured absorbance of resulting DNA samples to determine concentrations
- Confirmation of AtCRE
- Performed a digestion of AtCRE with EcoRI against a water control and a positive control (DQ2325601 digested with EcoRI)
- Ran product on a 1% gel for 40 minutes at 80V
- Two of six samples showed desired bands.
Matt
- Ligation of PTP2 with pSSA42
- Attempted another ligation of PTP2/pSSA42 this time with 1:1, 3:1, Vector with ligase, H2O, Vector without ligase.
