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Team:Valencia/Notebook/Protocols
From 2008.igem.org
Glycerol Stock
2 ml Eppendorf containing:
| Sterile Glycerol (stock 50 %) | 1ml |
| Half volume of a culture plate |
SD Medium
| Yeast Nitrogen Base | 0.67% |
| Glucose | 2% |
| Agar | 2% |
| Casamino acids | 0.1% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
SP liquid medium
| Yeast Nitrogen Base | 0.67% |
| Potassium phosohate | 0.1% |
| Ammonium sulfate | 0.12% |
| Glucose | 0.05% |
| Lactic acid | 2% |
| Casamino acids | 0.1% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
YPKAc Medium
| Yeast extract | 1% |
| Peptone | 2% |
| Potassium acetate | 2% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
