Preparation of linker_alpha (BBa_K103009)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + linker_alpha (BBa_K103009).

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr, Michał K.

1. Repetition of ligation of isolated DNA fragments: pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 30 September).
2. Transformation of TOP10 with above ligation.
3. Plating on LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 (with lactose promoter and OmpA_linker) + alpha2 to LB with kanamycin.