Team:Warsaw/Calendar-Main/1 August 2008

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Cloning of truncated fragment of protein A (ΔA)

Michał K.

  1. Colony PCR on pACYC177+OmpA_alpha + ΔA and pACYC177+OmpA_omega + ΔA colonies of tranformants.
  2. Gel electrophoresis of PCR products (Fig. 1.).

Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+ΔA and pACYC177_OmpA_omega+ΔA
Upper gel:
1. Marker
2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+ΔA
Lower gel:
1. Marker
2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+ΔA
12. positive control - pACYC_OmpA_A_alpha
13. negative control - pACYC_OmpA_Z_alpha



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