Team:Warsaw/Calendar-Main/24 May 2008

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Rifampicin test #2

Piotr

StrainInducer / repressorNumber of colonies on rifampicin
Top10none19
Top10 pMPM-T5-AIDnone10
Top10 pMPM-T5-AID0.1% Arabinose154
Top10 pTrc99a-AIDnone11
Top10 pTrc99a-AID0.5 mM IPTG59

Conclusion: AID seems to work!

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Isolation of hypothetical pMPM-T5 with transcriptional fusion from cultures.
  2. Isolation of hypothetical pMPM-T5 with translational fusion from cultures.
  3. Control digest of pMPM-T5 with transcriptional fusionwith EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translational fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (still no proper colonies found).

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