Team:Warsaw/Calendar-Main/8 August 2008

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Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)

Piotr:

  1. Inoculation of OmpA_omega_ΔA_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain.
  2. 5 hours culture
  3. Bacteria centrifuged and frozen.