Team:iHKU/Notebook

From 2008.igem.org

Home	The Team	The Project	Parts	Modeling	Notebook	Fun & Multimedia

Wet lab log book for the biobrick subgroup

(Many Thanks to MIT team for setting a good example for us ^_^)

Click on any day below to see what wet-lab procedures were conducted.

log book summary

0703 done: punched: and transformed. BBa_Q04510 BBa_R0062 BBa_R0011 BBa_I732096

Planning and summary section for the biobrick subgroup

here is the Biobrick subgroup progress,

Roy has got these following biobircks:

Part:BBa_E0240 Colony standard measurement for promotor

p1010 psb3K3

parts already obtained by peter：

Part:BBa_F2622 Colony

Part:BBa_T9002 Punch

Part:BBa_I731007 Punch

Part:BBa_B0030 Punch

S17 strain colony ready for ccdB

parts we further need to get

Part:BBa_Q04510 1013 7A Transf

Part:BBa_R0062 1000 4G Transf

Part:BBa_R0011 1000 4b Transf

BBa_I732096 1014 3G Transf reporter containing the standard GFP and LacZa

parts that may be useful

http://partsregistry.org/Part:BBa_B0031

http://partsregistry.org/Part:BBa_B0032

http://partsregistry.org/Part:BBa_B0033

http://partsregistry.org/Part:BBa_B0034

parts that we need to build:

1 Part:BBa_K094001CheZ assumed without rbs

2 Part:BBa_K094002LuxRI assumed with rbs and terminator

composite we need to construct:

1plaLuxRpluxCheZGFPLva BBa_F2622 BBa_B0030 BBa_K094001 BBa_B0030 BBa_E0022 BBa_B0015 （something wrong with GFPLva and i will change them later）

2plaLuxRpluxCIpldCheZGFPLva BBa_F2622 BBa_Q04510 BBa_B0030 BBa_K094001 BBa_E0430

3plaLuxRpluxLacZ BBa_F2622 BBa_B0030 BBa_I714901 BBa_B0015

4plaLuxRpluxCIpldLacZ BBa_F2622 BBa_Q04510 BBa_B0030 BBa_I714901 BBa_B0015

5plaLuxRIpluxCheZGFPLva BBa_R0011 BBa_K094002 BBa_R0062 BBa_B0030 BBa_K094001 BBa_E0430

6 first

7 Part:BBa_K094009

parts entered on 080822

Notebook

Retrieved from "http://en.wikipedia.org/wiki/User_talk:H0683090"