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Transforming Plasmid into Bacillus subtillis
From 2008.igem.org
- Pipette 10μl plasmid to be transformed into 2mL recovery tube.
- Add 400mL B. subtilis cells. (N.B. These cells must be prepped for transformation by nutrient starvation and heat stress.)
- Mix by inverting the tube 5 times.
- Incubate at 37C for 1 hour whilst shaking. To allow sufficient mixing tape the tubes to the rack and place the rack on its side on the shaking table (see diagram below).
