User:University of Washington/28 July 2008

From 2008.igem.org

LuxR from AraC and TetR

- On progress of deciding what plasmid to use as a vector for AraC and TetR promotor (after PCR from Elowitz's)

- Found out that MG1655z1 from Elowitz produced AraC, TetR, LacI, and LuxR. The gene for AraC, TetR, LacI were believed to be on the E.coli chromosome. LuxR gene is on the separated plasmid pACYC184 which has Tet and Cam resistance sites.

- To check the activity and existence of the plasmid/antibiotics, the E.coli strain from Elowitz containing plasmid pCS26 with D29 promoter(AraC and TetR..with KAN resistance) were inoculated onto Kan, Cam, and Tet LB+Agar plates.


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