Utah State/22 October 2008

From 2008.igem.org

Transformations from yesterday produced bacterial lawns. New ampicillin solution was made and used in new plates.

PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's instructions.