Utah State/8 October 2008

From 2008.igem.org

All liquid cultures from yesterday were turbid. 0.5 ml were saved for each possible biobrick for cryostocks. Plasmid DNA was isolated from the rest of each culture. Transformations were performed from the ligations from 10/6 (phbA into pSB1A3, phbA into pSB3K3, phbB into pSB1A3, and phbB into pSB3K3). To conserve competent cells, 50 ul competent cells were used for all four transformations (12ul/transformation) and 2 ul DNA was added to each.