Waterloo/5 September 2008

From 2008.igem.org

080905 Fri

[x] analyze Thursday's gel; select which parts to move forward with
[x] redo diag digest and gel of gene6 vs. putative gene6'
- gene6' looks too much larger than gene6 than it should
[x] full digest (3.5 h):
a) vector to clone pRecA
b) parts chosen to move forward with
c) synthesized parts
[x] gel extraction of a) and b) (2 - 2.5 h)
[x] ligation of a) with b) (0.5 - 1 h)

[] make media (2 h)

[] transformation of ligations and pDNA of synthesized parts
[] reorganize freezer boxes

Crossover PCR:
- design/order primers for:

  • gene6"
  • cI"
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