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Team:Hawaii/Notebook/2008-07- 2
From 2008.igem.org
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:* Restreaked colonies on fresh LB+anitbiotic plates | :* Restreaked colonies on fresh LB+anitbiotic plates | ||
| - | == Large-Scale Plasmid prep of BioBricks == | + | === Large-Scale Plasmid prep of BioBricks === |
:<strong> Margaret </strong> | :<strong> Margaret </strong> | ||
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|~1kb, maybe 5.7ng/uL | |~1kb, maybe 5.7ng/uL | ||
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| - | + | <strong>Future Work for this experiment</strong> | |
| + | *There is salt contamination in the plasmid preps. This is shown in the smeared bands. This occurred because the ethanol did not completely dry. We need to purify. | ||
===Remade seed stocks of cyanbacteria=== | ===Remade seed stocks of cyanbacteria=== | ||
:<strong>Krystle</strong> | :<strong>Krystle</strong> | ||
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= Discussion = | = Discussion = | ||
Latest revision as of 21:55, 7 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Checked for Biobrick transformants
- Grace
| Biobrick | Colonies |
|---|---|
| B0014 | none |
| B0015 | 4 |
| E0026 | 4 |
| E0240 | none |
| I14032 | none |
| J04430 | none |
- Restreaked colonies on fresh LB+anitbiotic plates
Large-Scale Plasmid prep of BioBricks
- Margaret
| Lane | Biobrick/Other | description |
|---|---|---|
| 1 | tri-dye ladder | very light, no difference in intensity |
| 2 | B0030 | 1.75kb, brighter than ladder, a lot of RNA |
| 3 | R0010 | 2.1kb, brighter than ladder, No RNA?? |
| 4 | C0012 | 2.5kb, brighter than ladder, a lot of RNA |
| 5 | E0040 | 2.4kb, brighter than ladder, a lot of RNA |
| 6 | J33207 | 2.5kb, maybe 5.7ng/uL, No RNA?? |
| 7 | rep region | 0.5kb, 5.7ng/uL |
| 8 | (+) control | bright band below 0.5kb |
| 9 | (-) control | all clear |
| 10 | SigE | ~1kb, maybe 5.7ng/uL |
Future Work for this experiment
- There is salt contamination in the plasmid preps. This is shown in the smeared bands. This occurred because the ethanol did not completely dry. We need to purify.
Remade seed stocks of cyanbacteria
- Krystle
Discussion
- Use commercial-grade competent cells for E0240 and I14032 extraction? We really need these two Biobricks.
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
