Team:Chiba/Receiver

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==Plasmid_Copy_number==
==Plasmid_Copy_number==
===Design===
===Design===
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レシーバーのコピーナンバーを減らすことで、thresholdに達するまでの時間を伸ばす。
 
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(小林)
 
===Experiment===
===Experiment===

Latest revision as of 20:40, 27 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements

Signal Molecule Receiver

Quorum_Sensing_Crosstalk

Design

Table.1 LuxR family gene

センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。(杉山)

Experiment

Plasmid_Copy_number

Design

Experiment

LuxR_mutant

Design

Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)


Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))

私たちはmutated Receiverを用いることで、AHL感受性の違う2種類(WTと変異体)のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)

Experiment

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements