Team:Warsaw/Calendar-Main/15 October 2008

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<h3>Preparation of <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a></h3>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3>
<h4>Piotr, Michał K.</h4>
<h4>Piotr, Michał K.</h4>
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<ol><li> Repetition of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> (from 30 September).</li>
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<ol><li> Repetition of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> (from <a href=https://2008.igem.org/Team:Warsaw/Calendar-Main/30_September_2008>30 September</a>).</li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li>
<li> Plating on LB with kanamycin.</li></ol>
<li> Plating on LB with kanamycin.</li></ol>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3>
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<h4>Michał K.</h4>
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<p> Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a> to LB with ampicillin. </p>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103017>OmpA_linker_alpha_linker under Plac (BBa_K103017)</a></h3>
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<h3>Preparation of BioBricks</h3>
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<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<ol><li> Inoculation of colonies from plate with ligation of 3kb(?????)+ link_alpha, 3kb(????)_pLac_OmpA_ + alpha2 LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin. </li>
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<p> Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> (with lactose promoter and OmpA_linker) + alpha2 to LB with kanamycin. </p>
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Latest revision as of 23:46, 27 October 2008

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Preparation of linker_alpha (BBa_K103009)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + linker_alpha (BBa_K103009).

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr, Michał K.

  1. Repetition of ligation of isolated DNA fragments: pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 30 September).
  2. Transformation of TOP10 with above ligation.
  3. Plating on LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 (with lactose promoter and OmpA_linker) + alpha2 to LB with kanamycin.

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