Team:Hawaii/Notebook/2008-07-21

From 2008.igem.org

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:*continued from [[Team:Hawaii/Notebook/2008-07-19|Saturday]], ran a gel
:*continued from [[Team:Hawaii/Notebook/2008-07-19|Saturday]], ran a gel
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===Restriction Digest===
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:<strong>Krystle</strong>
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:*Performed a 2 separate restriction digests on nir, gfp, and gfp fusion brick with EcoRI and SpeI
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::''trial 1:'' digested 5ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 3 hours
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::''trial 2:'' digested 25ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 2 hours
== Drylab Work ==
== Drylab Work ==

Revision as of 04:18, 22 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Colony PCR

Margaret
  • Colony PCR of pSB1A2, pSB1A3, pSB3K3
Lane 1 contains the 2-log ladder, lane 2: pSB1A3's ccdB insert, lane3: pSB1A2's ccdB insert, lane 4: pSB3K3's ccdB insert, lane 5: positive control, lane 6: negative control, lane 7: pSMC121.

PCR amplification of pRL1383a parts

Restriction Digest

Krystle
  • Performed a 2 separate restriction digests on nir, gfp, and gfp fusion brick with EcoRI and SpeI
trial 1: digested 5ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 3 hours
trial 2: digested 25ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 2 hours

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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