Team:Hawaii/Notebook/2008-08-17
From 2008.igem.org
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{| class=wikitable border=1 align=center | {| class=wikitable border=1 align=center | ||
! DNA | ! DNA | ||
+ | ! Plate | ||
! Total colony forming units | ! Total colony forming units | ||
! Blue colonies | ! Blue colonies | ||
|- | |- | ||
| align=center|BB-pRL1383a + J33207 (plated on IPTG + X-gal) | | align=center|BB-pRL1383a + J33207 (plated on IPTG + X-gal) | ||
- | | align=center| | + | | align=center|LB + sp<sub>100</sub> + sm<sub>50</sub> + IPTG (1mM) + X-gal (2mM) |
+ | |align=center| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| align=center|BB-pRL1383a + J33207 (plated on X-gal) | | align=center|BB-pRL1383a + J33207 (plated on X-gal) | ||
+ | |align=center|LB + sp<sub>100</sub> + X-gal (2mM) | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
|- | |- | ||
|align=center| BB-pRL1383a (negative control) | |align=center| BB-pRL1383a (negative control) | ||
+ | |align=center|LB + sp<sub>100</sub> + sm<sub>50</sub> + IPTG (1mM) + X-gal (2mM) | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| align=center|no DNA (negative control) | | align=center|no DNA (negative control) | ||
+ | |align=center|LB + sp<sub>100</sub> + sm<sub>50</sub> + IPTG (1mM) + X-gal (2mM) | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| |
Revision as of 01:58, 17 August 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
DNA | Plate | Total colony forming units | Blue colonies |
---|---|---|---|
BB-pRL1383a + J33207 (plated on IPTG + X-gal) | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) | ||
BB-pRL1383a + J33207 (plated on X-gal) | LB + sp100 + X-gal (2mM) | ||
BB-pRL1383a (negative control) | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) | ||
no DNA (negative control) | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) |
- Colony PCR to verify insert
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]