From 2008.igem.org
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| - | ８月の実験ログは[[Team:Chiba/notebook/input/august|こちら]]
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| - | == 2008.9.1 ==
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| - | ・Ptet＋RBS＋cIの、Mini Prep産物の濃度チェック。
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| - | <BR>-Ptet-cI-pMB1-Amp-の機能チェック。
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| - | <BR>-Ptet-cI-pMB1-Amp-,-PcI-GFP-p15A-Cm-をDouble Transformation(BWΔflic)
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| - | <BR>結果→GFPが発現していた。
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| - | <BR>→-PcI-GFP-p15A-Cm-が機能していないのでは？
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| - | <BR>→ターミネーターをつける、pSB1A3にのせかえる。
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| - | <BR>-Ptet-cI-pMB1-Amp-のDigestion Check
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| - | <BR>混ぜ表
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| - | <table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <td width="257"></td>
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| - | <td>Double</td><td>Single</td>
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| - | <tr>
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| - | <td>dH<sub>2</sub>O</td>
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| - | <td>1</td><td>2</td>
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| - | </tr>
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| - | <tr>
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| - | <td>XbaI</td>
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| - | <td>1</td><td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>SpeI</td>
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| - | <td>1</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>BSA(×10)</td>
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| - | <td>1</td><td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>NEB(×10)</td>
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| - | <td>1</td><td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>DNA</td>
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| - | <td>5</td><td>5</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>10</td><td>10</td>
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| - | </tr>
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| - | </table>
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Latest revision as of 01:57, 24 September 2008
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