Team:Freiburg Transfection
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<h3>'''Testing the transfectionvector-CMV promoter construct'''</h3> | <h3>'''Testing the transfectionvector-CMV promoter construct'''</h3> | ||
- | To test the functionality of the transfectionvector and the CMV-promoter, | + | To test the functionality of the transfectionvector and the CMV-promoter, YFP was cloned behind the promoter and the plasmid was brought into 293T cells. The detection of YFP took place 1 day later under a microscope with YFP filter.<br> |
The transfected cells show fluorescence by excitation of 510-520nm while the untransfected remain dark at this wavelength <br> | The transfected cells show fluorescence by excitation of 510-520nm while the untransfected remain dark at this wavelength <br> | ||
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[[Image:Freiburg2008_150%_293Tzvi.jpg]] [[Image:Freiburg2008_Kontrolle.jpg]] [[Image:Freiburg2008_Kontrolle_Durchlicht.jpg]]<br> | [[Image:Freiburg2008_150%_293Tzvi.jpg]] [[Image:Freiburg2008_Kontrolle.jpg]] [[Image:Freiburg2008_Kontrolle_Durchlicht.jpg]]<br> | ||
- | ''Figure 1_Transfection''<br> | + | ''Figure 1_Transfection: transfected 293T cells (left), untransfected cells (middle) and transfected cells transmitted light (right)''<br> |
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<h3>'''Localization at the cell membrane'''</h3> | <h3>'''Localization at the cell membrane'''</h3> |
Revision as of 17:39, 28 October 2008