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Virginia/8 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==Goals== *Prepare overnight broth of successful single colony of Promoter + RBS transformation *Plate Screening plasmid (psb1a10) when it arrives from iGem *Try again with ligation of RFP...) |
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==Goals== | ==Goals== | ||
| - | *Prepare overnight broth of | + | *Prepare overnight broth of single successful colony of Promoter + RBS transformation |
*Plate Screening plasmid (psb1a10) when it arrives from iGem | *Plate Screening plasmid (psb1a10) when it arrives from iGem | ||
*Try again with ligation of RFP and GFP enzyme cut DNA | *Try again with ligation of RFP and GFP enzyme cut DNA | ||
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*Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation | *Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation | ||
*Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon | *Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon | ||
| - | |||
==Notes== | ==Notes== | ||
Revision as of 18:01, 8 July 2008
Goals
- Prepare overnight broth of single successful colony of Promoter + RBS transformation
- Plate Screening plasmid (psb1a10) when it arrives from iGem
- Try again with ligation of RFP and GFP enzyme cut DNA
- Transform this ligation and pray that it grows in the incubator
- Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
- Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon
Notes
- Maxiprepped bba_B0015 and bba_B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA
