Team:Johns Hopkins/Protocols

From 2008.igem.org

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[[Media:JHU_0708_Jaime-protocols.pdf|PCR Protocols from Jaime]]
[[Media:JHU_0708_Jaime-protocols.pdf|PCR Protocols from Jaime]]
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== Papers ==
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== Important Papers ==
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[[Media:JHU_0708_paper_Aregularoryheirarchy.pdf|1]]  Herskowitz I. <i> A regulatory hierarchy for cell specialization in yeast. </i> (REVIEW.) Nature. 342:749-757 (1989)<br>
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[[Media:JHU_0708_paper_Aregularoryheirarchy.pdf| *d]]  Herskowitz I. <i> A regulatory hierarchy for cell specialization in yeast. </i> (REVIEW.) Nature. 342:749-757 (1989)<br>
[[Media:JHU_0708_paper_MolecularMechanismofcell-typedeterminationinbuddingyeast.pdf|1]] Johnson AD. <i> Molecular mechanisms of cell-type determination in budding yeast. </i>(REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995)<br>
[[Media:JHU_0708_paper_MolecularMechanismofcell-typedeterminationinbuddingyeast.pdf|1]] Johnson AD. <i> Molecular mechanisms of cell-type determination in budding yeast. </i>(REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995)<br>
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[[Media:JHU_0708_paper_Awalk.pdf|1]]  Bardwell L. <I>A walk-through of the yeast mating pheromone response pathway. (REVIEW.)</I> Peptides. 25:1465-1476 (2004)<br>
[[Media:JHU_0708_paper_Awalk.pdf|1]]  Bardwell L. <I>A walk-through of the yeast mating pheromone response pathway. (REVIEW.)</I> Peptides. 25:1465-1476 (2004)<br>
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=== Yeast Vector Papers ===
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[[Media:JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf|**]]Broach JR. <I>Transformation in yeast: development of a hybrid cloning vector and isolation of the </I>CAN1<I> gene.</I> Gene. 8(1):121-133 (1979)< br>
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[[Media:JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf|**]]
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Stearns, T. <I>Manipulating yeast genome using plasmid vectors. (REVIEW.)</I> Methods in enzymology. 185:280-297 (1990)<br>
[[Media:JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf|1]]  Hill JE <I>et al.</I> <I>Yeast/</I>E. coli<I> Shuttle Vectors with Multiple Unique Restriction Sites.</I> Yeast. 2:163-167 (1986)<br>
[[Media:JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf|1]]  Hill JE <I>et al.</I> <I>Yeast/</I>E. coli<I> Shuttle Vectors with Multiple Unique Restriction Sites.</I> Yeast. 2:163-167 (1986)<br>
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[[Media:JHU_0708_paper_Yeast_vectors_for_the_controlled_expression_of_heterologous_proteins.pdf|JHU_0708_paper_Yeast_vectors_for_the_controlled_expression_of_heterologous_proteins.pdf]]<br>
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[[Media:JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf|**]]
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Sikorski RS. <I>A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in </I>Saccharomyces cerevisiae. Genetics. 122:19-27 (1989)<br>
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[[Media:JHU_0708_paper_Yeast_vectors_for_the_controlled_expression_of_heterologous_proteins.pdf|***]]
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Mumberg D <I>et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds.</I> Gene. 156:119-122 (1995)<br>
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=== Other ===
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[[Media:JHU_0708_paper_TransfofYeast.pdf|JHU_0708_paper_TransfofYeast.pdf]]<br>
[[Media:JHU_0708_paper_TransfofYeast.pdf|JHU_0708_paper_TransfofYeast.pdf]]<br>
[[Media:JHU_0708_paper_Three-prongedgenomicanalysis.pdf|JHU_0708_paper_Three-prongedgenomicanalysis.pdf]]<br>
[[Media:JHU_0708_paper_Three-prongedgenomicanalysis.pdf|JHU_0708_paper_Three-prongedgenomicanalysis.pdf]]<br>
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[[Media:JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf|JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf]]<br>
 
[[Media:JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf|JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf]]<br>
[[Media:JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf|JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf]]<br>
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[[Media:JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf|JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf]]<br>
 
[[Media:JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf|JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf]]<br>
[[Media:JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf|JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf]]<br>
[[Media:JHU_0708_paper_GlobalTranscriptionRegulators.pdf|JHU_0708_paper_GlobalTranscriptionRegulators.pdf]]<br>
[[Media:JHU_0708_paper_GlobalTranscriptionRegulators.pdf|JHU_0708_paper_GlobalTranscriptionRegulators.pdf]]<br>
[[Media:JHU_0708_paper_ForeignGeneExpression.pdf|JHU_0708_paper_ForeignGeneExpression.pdf]]<br>
[[Media:JHU_0708_paper_ForeignGeneExpression.pdf|JHU_0708_paper_ForeignGeneExpression.pdf]]<br>
[[Media:JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf|JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf]]<br>
[[Media:JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf|JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf]]<br>
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[[Media:JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf|JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf]]<br>
 
[[Media:JHU_0708_paper_BentDNA.pdf|JHU_0708_paper_BentDNA.pdf]]<br>
[[Media:JHU_0708_paper_BentDNA.pdf|JHU_0708_paper_BentDNA.pdf]]<br>
[[Media:JHU_0708_paper_Aregularoryheirarchy.pdf|JHU_0708_paper_Aregularoryheirarchy.pdf]]<br>
[[Media:JHU_0708_paper_Aregularoryheirarchy.pdf|JHU_0708_paper_Aregularoryheirarchy.pdf]]<br>

Revision as of 17:06, 13 July 2008


Contents

Protocols

Plasmid Miniprep
Ligation and Transformation
PCR Protocols from Jaime

Important Papers

*d Herskowitz I. A regulatory hierarchy for cell specialization in yeast. (REVIEW.) Nature. 342:749-757 (1989)

1 Johnson AD. Molecular mechanisms of cell-type determination in budding yeast. (REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995)

1 Hu R-G et al. The N-end rule pathway as a nitric oxide sensor controlling the levels of multiple regulators. Nature. 437:981-986 (2005)

1 Varshavsky A. The N-end Rule pathway of protein degradation. (REVIEW.) Genes to Cells. 2:13-28 (1997)

1 Sheff MA & Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 21:661-670 (2004)

1 Bardwell L. A walk-through of the yeast mating pheromone response pathway. (REVIEW.) Peptides. 25:1465-1476 (2004)

Yeast Vector Papers

**Broach JR. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene. 8(1):121-133 (1979)< br>

** Stearns, T. Manipulating yeast genome using plasmid vectors. (REVIEW.) Methods in enzymology. 185:280-297 (1990)

1 Hill JE et al. Yeast/E. coli Shuttle Vectors with Multiple Unique Restriction Sites. Yeast. 2:163-167 (1986)

** Sikorski RS. A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19-27 (1989)

*** 

Mumberg D et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene. 156:119-122 (1995)

Other

JHU_0708_paper_TransfofYeast.pdf
JHU_0708_paper_Three-prongedgenomicanalysis.pdf
JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf
JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf
JHU_0708_paper_GlobalTranscriptionRegulators.pdf
JHU_0708_paper_ForeignGeneExpression.pdf
JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf
JHU_0708_paper_BentDNA.pdf
JHU_0708_paper_Aregularoryheirarchy.pdf

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