Team:Hawaii/Notebook/2008-07-21

From 2008.igem.org

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(Restriction Digest)
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===Name of Task===
===Name of Task===
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:<strong> name of person/people who performed the task</strong>
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:<strong> Krystle, Margaret</strong>
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
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:* We figured out why some of the biobrick extractions did not transform. Some of these parts are on pSB2K3[[http://partsregistry.org/wiki/index.php?title=Part:pSB2K3]] which is a low copy until IPTG is added to the medium in which case it becomes high copy! So next time we make this, add 1mM IPTG to the media.
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:* e.g. read through papers, worked on proposal, etc.
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Revision as of 04:23, 22 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Colony PCR

Margaret
  • Colony PCR of pSB1A2, pSB1A3, pSB3K3
Lane 1 contains the 2-log ladder, lane 2: pSB1A3's ccdB insert, lane3: pSB1A2's ccdB insert, lane 4: pSB3K3's ccdB insert, lane 5: positive control, lane 6: negative control, lane 7: pSMC121.

PCR amplification of pRL1383a parts

Restriction Digest

Krystle
  • Performed 2 restriction digests on nir, gfp, and gfp fusion brick with EcoRI and SpeI
trial 1: digested 5ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 3 hours
trial 2: digested 25ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 2 hours

Drylab Work

Name of Task

Krystle, Margaret
  • We figured out why some of the biobrick extractions did not transform. Some of these parts are on pSB2K3http://partsregistry.org/wiki/index.php?title=Part:pSB2K3 which is a low copy until IPTG is added to the medium in which case it becomes high copy! So next time we make this, add 1mM IPTG to the media.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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