Edinburgh/27 July 2008
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- | * Result of transformations: no growth for 'L12' (L19), 'L15' (L20) or 'L28' (L21). For 'L29' (L22) there are some blue and white colonies. For 'L31' (L23) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For the ''crtE'' ligation, there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF) | + | </style> |
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+ | {| align="left" | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
+ | |} | ||
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+ | :::: '''[[Edinburgh/26_July_2008|< Previous Entry]]''' | ||
+ | |||
+ | == Week 6 == | ||
+ | === Sunday 27 July 08 === | ||
+ | |||
+ | * Result of transformations: no growth for 'L12' (L19), 'L15' (L20) or 'L28' (L21). For 'L29' (L22) there are some blue and white colonies. For 'L31' (L23) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For the ''crtE'' ligation, there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)<br /> | ||
+ | <br /> | ||
+ | |||
+ | :::: '''[[Edinburgh/28_July_2008|Next Entry >]]''' |
Revision as of 11:57, 26 August 2008
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Week 6
Sunday 27 July 08
- Result of transformations: no growth for 'L12' (L19), 'L15' (L20) or 'L28' (L21). For 'L29' (L22) there are some blue and white colonies. For 'L31' (L23) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For the crtE ligation, there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)