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Edinburgh/28 July 2008
From 2008.igem.org
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Week 7
Monday 28 July 08
- Repeated transformation of L20 (pSB1A2+rbs+appY) and L21 (pSB1A2+rbs+crtB), and plated on plates 63-66. (Yan, Omar).
- PCR of cenA, cenB, cenC and cex from cell suspension (P38-P41; cell solution incubated at 73-80°C for 8 minutes to kill cells/inactivate DNAse) and DNA solution 'purified' from contaminated cell culture (P42-P45). Denaturing 95°C for 1 min, annealing 55°C for 10s, extension 70°C for 90s. PCR products run on Gel 26. (AM)
- Prepared L25 of P36 (glgC-mut1,2 PCRed with primers f2 and r2) and Edinbrick1. (AM)
- Minipreps M49-M54 of possible pSB1A2+rbs+crtI transformants. (CF)
