Team:Warsaw/Calendar-Main/9 May 2008

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<html><h3>Preparation of pMPMT5+AID construct</h3>
<h4>Michał K.:</h4>
<h4>Michał K.:</h4>

Revision as of 08:53, 5 October 2008

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Preparation of pMPMT5+AID construct

Michał K.:

  1. Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
  2. DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp).
  3. Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
  4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp).
  5. Electrophoresis to estimate the concentration of isolated DNA.
  6. Ligation of isolated DNA fragments.





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