"
Team:Warsaw/Calendar-Main/24 May 2008
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
{{WarNotebook}} | {{WarNotebook}} | ||
<!-- do not edit above me! --> | <!-- do not edit above me! --> | ||
| - | <html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3> | + | <html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p><ol> | <p><ol> | ||
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with transcriptional fusion from cultures.</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPM-T5 with transcriptional fusion</a> from cultures.</li> |
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translational fusion from cultures.</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPM-T5 with translational fusion</a> from cultures.</li> |
| - | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcriptional fusion with EcoRI (EcoRI buffer).</li> | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPM-T5 with transcriptional fusion</a>with EcoRI (EcoRI buffer).</li> |
| - | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translational fusion with BamHI (BamHI buffer).</li> | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPM-T5 with translational fusion</a> with BamHI (BamHI buffer).</li> |
<li> Gel electrophoresis (still no proper colonies found).</li> | <li> Gel electrophoresis (still no proper colonies found).</li> | ||
Revision as of 16:32, 12 October 2008
 |
|
||||||||
 |
|
||||||||
Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7Michał K.
|
|
