Team:Hawaii/Notebook/2008-10-23

From 2008.igem.org

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===Verification of transformants===
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[[Image:102308colonyPCR.png|right|thumb|500px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each lane.]]
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= Discussion =
= Discussion =

Revision as of 21:45, 23 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Antibiotic test

Grace
  • No change from yesterday
  • Colony PCR of 6 colonies from sp40, 50, 75 and 8 colonies from sp100 plates

Verification of transformants

EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each lane.
Grace
Colony count of transformants
Plasmid Colonies
J04430 0
(-) on amp100 0
BB-1 4
(-) on sp100 0
  • Colony PCR of 4 BB-1 transformants

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]