Team:NTU-Singapore/Notebook/18 July 2008

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(New page: =Friday 18 July= *RESULTS FROM THURSDAY TRANSFORMATION: **Plate of E7 got 4 colonies!! **Plate of T7ptag got 8 colonies!! *We also received LsrA and Lysis (lyophilized form) in Bluescript ...)
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=Friday 18 July=
=Friday 18 July=
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*RESULTS FROM THURSDAY TRANSFORMATION:
*RESULTS FROM THURSDAY TRANSFORMATION:
**Plate of E7 got 4 colonies!!
**Plate of E7 got 4 colonies!!
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**1600: transform lysis and LsrA (0.3ul each) into competent cells.
**1600: transform lysis and LsrA (0.3ul each) into competent cells.
**1700: inoculate 4 colonies of E7 and 8 of T7ptag.
**1700: inoculate 4 colonies of E7 and 8 of T7ptag.
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Revision as of 12:16, 25 October 2008


Friday 18 July

  • RESULTS FROM THURSDAY TRANSFORMATION:
    • Plate of E7 got 4 colonies!!
    • Plate of T7ptag got 8 colonies!!
  • We also received LsrA and Lysis (lyophilized form) in Bluescript vector. We eluted them using TE buffer.
  • Afternoon:
    • 1600: transform lysis and LsrA (0.3ul each) into competent cells.
    • 1700: inoculate 4 colonies of E7 and 8 of T7ptag.