Team:Warsaw/Calendar-Main/15 October 2008

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<h3>Preparation of <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a></h3>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3>
<h4>Piotr, Michał K.</h4>
<h4>Piotr, Michał K.</h4>
<ol><li> Repetition of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> (from 30 September).</li>
<ol><li> Repetition of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> (from 30 September).</li>

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Preparation of linker_alpha (BBa_K103009)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + linker_alpha (BBa_K103009).

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr, Michał K.

  1. Repetition of ligation of isolated DNA fragments: pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 30 September).
  2. Transformation of TOP10 with above ligation.
  3. Plating on LB with kanamycin.

Preparation of BioBricks

Michał K.

  1. Inoculation of colonies from plate with ligation of 3kb(?????)+ link_alpha, 3kb(????)_pLac_OmpA_ + alpha2 LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin.

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