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Team:Chiba/parts/BBa K084008
From 2008.igem.org
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====Purpose==== | ====Purpose==== | ||
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity. | To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity. | ||
| - | + | ====Method==== | |
#Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908). | #Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908). | ||
#Inoculated them independently in liquid media. Incubated at 37°C 12h | #Inoculated them independently in liquid media. Incubated at 37°C 12h | ||
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#Incubated at 30°C. | #Incubated at 30°C. | ||
#Measured intensity of green fluorescence at regular time intervals. | #Measured intensity of green fluorescence at regular time intervals. | ||
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====[[Team:Chiba/protocol/phenotype/timedelay|crosstalk experients]]==== | ====[[Team:Chiba/protocol/phenotype/timedelay|crosstalk experients]]==== | ||
#E.Coli strain JW1908 or XL10Gold. | #E.Coli strain JW1908 or XL10Gold. | ||
Revision as of 11:39, 28 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
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parts usage
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)])
日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
English:
Experient
Purpose
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
Method
- Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.
crosstalk experients
- E.Coli strain JW1908 or XL10Gold.
- Reaction Temparature 37 or 30.
- Cell number.
Results
Fig.
