_parts
All of our parts feature our expanded Pre- and Suffix for in frame cloning of fusion proteins [http://parts.mit.edu/igem07/index.php/Freiburg07/report_fusion_parts (more)]. A complete, detailed list of all parts submitted to the registry in this year´s project can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Freiburg here], as well as the respective DNA-Sequences.
basic parts
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157001 BBa_K157001]
Description:
This sequence mediates transportation of the protein to a translocational pore by an RNA–multiprotein complex, the signal recognition particle (SRP) when fused to the n-terminus of a fusion protein with a transmembrane region[1].
There, after a pause of translation, the signal sequence is released and translation and translocation of the nascent chain are restarted[2].
Source:
Human EGFR (ErbB-1) signal sequence; originally mediating membrane integration of the EGF-receptor (ErbB-1). Sequence taken from UniProtKB/Swiss-Prot entry P00533.
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
References:
[1] Walter P, Johnson AE: “Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane.” Annu Rev Cell Biol 1994, 10:87-119
[2] Robert M Stroud, Peter Walter: “Signal sequence recognition and protein targeting”, Current Opinion in Structural Biology 1999, 9:754–759
AA sequence:
MRPSGTAGAA LLALLAALCP ASRA
gene sequence:
ATGAGACCAT CTGGTACTGC TGGAGCCGCA TTGCTGGCAC TTTTGGCTGC GCTGTGCCCT GCAAGCAGAG CA
- Erbb1-transmembrane region
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157002 BBa_K157002]
Description:
Helical, single-span transmembrane region of the human EGF-Receptor type ErbB-1, sequence taken from UniProtKB/Swiss-Prot entry P00533.
source:
Transmembrane region of the human EGF-Receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533.
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA sequence:
ATGMVGALLL LLVVALGIGL FM
gene sequence:
ATAGCTACCG GAATGGTGGG TGCACTTTTG CTCCTTTTGG TCGTTGCCCT GGGGATAGGA CTCTTTATG
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157003 BBa_K157003]
Description:
Singlechain Fv.Fragment of the Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.
source:
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References:
[1]Ana Cumano and Klaus Rajewski: “Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP”, The EMBO Journal vol. 5 no.10 pp. 2459-2468, 1986
[2]D. Allen, T. Simon, F. Sablitzky, K. Rajewski and A. Cumano: “Antibody engineering for the analysis of affinity maturation of an anti-hapten response”, The EMBO Journal vol. 7 no.7 pp. 1995-2001, 1988
AASequence:
QVQLQQPGAE LVKPGASVKL SCKASGYTFT SYWMHWVKQR PGRGLEWIGR IDPNSGGTKY NEKFKSKATL TVDKPSSTAY MQLSSLTSED SAVYYCARYD YYGGSYFDYW GQGTTVTVSS GGGGSGGGGS GGGGSQAVVT QESALTTSPG ETVTLTCRSS TGAVTTSNYA NWVQEKPDHL FTGLIGGTNN RAPGVPARFS GSLIGDKAAL TITGAQTEDE AIYFCALWYS NHWVFGGGTK LTVLG
gene sequence:
CAGGTGCAGC TCCAGCAGCC CGGAGCCGAA CTGGTGAAGC CAGGCGCCAG CGTGAAGCTG TCCTGCAAGG CCAGCGGCTA CACCTTCACC AGCTACTGGA TGCACTGGGT GAAACAGAGG CCCGGCAGAG GCCTGGAATG GATCGGCCGG ATCGACCCCA ACAGCGGCGG CACCAAGTAC AACGAGAAGT TCAAGAGCAA GGCCACCCTG ACCGTGGACA AGCCCAGCAG CACCGCCTAC ATGCAGCTGT CCAGCCTGAC CAGCGAGGAC AGCGCCGTGT ACTACTGCGC CAGATACGAC TACTACGGCG GCAGCTACTT CGACTACTGG GGCCAGGGCA CCACCGTGAC CGTGTCCTCT GGGGGAGGGG GCTCAGGAGG AGGAGGAAGC GGGGGAGGGG GCAGCCAGGC CGTGGTGACC CAGGAAAGCG CCCTGACCAC TCCCCTGGCG GACAGTGACC TGACCTGCCG TCCTCTACAG GCGCCGTGAC CACAAGCAAC TACGCCAACT GGGTGCAGGA AAAGCCCGAC CACCTGTTCA CCGGCCTGAT CGGCGGCACA AACAACAGAG CCCCTGGCGT GCCCGCTAGA TTCAGCGGCA GCCTGATCGG GGACAAGGCC GCCCTGACAA TCACAGGCGC CCAGACCGAG GACGAGGCCA TCTACTTTTG CGCCCTGTGG TACAGCAACC ACTGGGTGTT CGGCGGAGGG ACCAAGCTGA CCGTGCTGGG C
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157004 BBa_K157004]
Description:
Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra[1,2].
Source:
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References:
[1] Gerald Beste, Frank S. Schmidt, Thomas Stibora and A. Skerra: “Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold“,Proc. Natl. Acad. Sci. USA Vol. 96, pp. 1898–1903, March 1999 Biochemistry
[2]Ingo P. Korndörfer, Gerald Beste and A. Skerra: “Crystallographic Analysis of an “Anticalin” With Tailored Specificity for Fluorescein Reveals High Structural Plasticity of the Lipocalin Loop Region”, PROTEINS: Structure, Function, and Bioinformatics 53:121–129 (2003)
AA sequence:
MAGDVYHDGA CPEVKPVDNF DWSQYHGKWW EVAKYPSPNG KYGKCGWAEY TPEGKSVKVS RYDVIHGKEY FMEGTAYPVG DSKIGKIYHS RTVGGYTRKT VFNVLSTDNK NYIIGYSCRY DEDKKGHWDH VWVLSRSMVL TGEAKTAVEN YLIGSPVVDS QKLVYSDFSE AACKVNNTG
gene sequence:
GACGTGTACC ACGACGGCGC CTGCCCCGAA GTGAAGCCCG TGGACAACTT CGACTGGTCC CAGTACCACG GCAAGTGGTG GGAGGTGGCC AAGTACCCCA GCCCCAACGG CAAGTATGGC AAGTGCGGCT GGGCCGAGTA CACCCCCGAG GGCAAGAGCG TGAAGGTGTC CAGATACGAC GTGATCCACG GCAAAGAATA CTTCATGGAA GGCACCGCCT ACCCCGTGGG CGACAGCAAG ATCGGCAAGA TCTACCACAG CCGGACCGTG GGCGGCTACA CCAGAAAGAC CGTGTTCAAC GTGCTGTCCA CCGACAACAA GAACTACATC ATCGGCTACA GCTGCCGCTA CGACGAGGAC AAGAAGGGCC ACTGGGACCA CGTGTGGGTG CTGTCCCGGT CCATGGTGCT GACCGGCGAG GCCAAGACCG CCGTGGAGAA CTACCTGATC GGCAGCCCCG TGGTGGACAG CCAGAAACTG GTGTACAGCG ACTTCAGCGA GGCCGCCTGC AAAGTGAACA AC
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157005 BBa_K157005]
Description:
C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant “Cerulean”, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP “Cerulean”, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein “Venus”[4] (Part Bba_I757008) results in green fluorescence[2].
AASequence:
KNGIKANFKI RHNIEDGSVQ LADHYQQNTP IGDGPVLLPD NHYLSTQSKL SKDPNEKRDH
gene sequence:
MVLLEFVTAA GITLGMDELY KAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGATG GCAGCGTGCA GCTGGCCGAT CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CTGGGCATGG ACGAGCTGTA CAAG
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157006 BBa_K157006]
Description:
N-terminal fragment (Amino acids1-155) of the monomeric[5] cyan fluorescent protein variant “Cerulean”, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-cCFP (Part Bba_K157005) generates the complete, working CFP “Cerulean”, whereas combination with the C-terminal fragment (Part Bba_K157007) of the monomeric yellow fluorescent Protein “Venus”[4] (Part Bba_I757008) results in green fluorescence[2].
AASequence:
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTLGVQCFA RYPDHMKRHD FFKSAMPEGY VQERTIFFKD DGNYKTRAEV KFEGDTLVNR IELKGIDFKE DGNILGHKLE YNAISDNVYI TADKQ
gene sequence:
ATGAGCAAGG GCGAGGAACT GTTCACCGGC GTGGTGCCCA TCCTGGTGGA GCTGGACGGC GACGTGAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AAGGCGACGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG GTGACCACCC TGGGCGTGCA GTGCTTCGCC AGATACCCCG ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACACT GGTGAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAATA TCCTGGGCCA CAAGCTGGAA TACAACGCCA TCAGCGACAA CGTGTACATC ACCGCCGACA GCAG
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157007 BBa_K157007]
Description:
C-terminal fragment (Amino acids157-237) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157008 (Split-Venus-nYFP) generates the complete, working YFP “Venus”, whereas combination with the n-terminal fragment (Part Bba_K157006) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].
AASequence:
KNGIKANFKI RHNIEDGGVQ LADHYQQNTP IGDGPVLLPD NHYLSYQSKL SKDPNEKRDH MVLLEFVTAA GITHGMDELY K
gene sequence:
AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACA TCGAGGACGG CGGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GAGCAAGCTG TCCAAGGACC CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTTGT GACAGCCGCC GGAATCACCC ACGGCATGGA CGAGCTGTAC AAG
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157008 BBa_K157008]
Description:
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP “Venus”, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].
AASequence:
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKLICT TGKLPVPWPT LVTTLYLQCF ARYPDHMKQH DFFKSAMPEG YVQERTIFFK DDGNYKTRAE VKFEGDTLVN RIELKGIDFK EDGNILGHKL EYNYNSHNVY ITADKQ
gene sequence:
ATGGTGTCCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAGCTGGAC GGCGACGTGA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAAGGCGA CGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTGGTGACCA CCCTGTACCT CCAGTGCTTC GCCAGATACC CCGACCACAT GAAGCAGCAC GATTTCTTCA AGAGCGCCAT GCCCGAGGGC TACGTGCAGG AACGGACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CAGAGCCGAA GTGAAGTTCG AGGGCGACAC ACTGGTGAAC CGGATCGAGC TGAAGGGCAT CGACTTCAAA GAGGACGGCA ATATCCTGGG CCACAAGCTG GAATACAACT ACAACAGCCA CAACGTGTAC ATCACCGCCG ACAAGCAG
Source (all split-fluorophores):
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References (all split-fluorophores):
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
-[4]Nagai, T. et al. “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications” J. Biol. Chem. 276, 29188-29194, 2001
-[5]Roger Y. Tsien et al. „Creating new fluorescent probes for cell biology“, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157009 BBa_K157009]
description:
Originally, this linker was used for fusion to the N-terminus of the C-terminal half of split fluorophores: Protein interactions can be examined by BiFC[1] using complementary, non-fluorescent fragments of fluorophores[2]. Therefore, it is essential that the C-terminal fragment of the fluorophore is not restricted too much in its mobility. The linker allows orientation and adaption of the C-terminal fragment to the N-terminal fragment of the split fluorophore and, thus, the reassembly of a working fluorescent protein. It has already been used in BiFC assays and is known to serve this purpose well[3]; anyway, another, more flexible linker that could be used instead is our “GGGGS-linker” (Part Bba_K157010).
References:
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
source:
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA Sequence:
RPACKIPNDL KQKVMNH
gene sequence:
CGACCAGCCT GTAAGATTCC AAATGACCTG AAGCAGAAAG TTATGAATCA C
- GGGGS-LINKER_BCR-Transmem.
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157010 BBa_K157010]
Description:
Helical single-span transmembrane region of the B-Cell-Receptor with a flexible 15 aa Linker at the N-terminus. Designed for fusion to proteins or peptides that are to be presented at the cells surface; signal peptide for membrane integration (e.g. part Bba_K157001) required at the N-terminus of the whole construct!
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157011 BBa_K157011]
See Part BBa_I757013 (Freiburg 2007)
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157012 BBa_K157012]
See Part BBa_I757014 (Freiburg 2007)
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157013 BBa_K157013]
Description:
A flexible, 15 aa long linker designed for fusion to/of proteins or peptides via in frame cloning. Consists of Glycine and Serine only.
source:
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA sequence:
GGGSGGGSGG GSGGG
gene sequence:
GGTGGAGGAG GTTCTGGAGG CGGTGGAAGT GGTGGCGGAG GTAGC
composite parts
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157014 BBa_K157014]
Description:
C-terminal part of the Split fluorophore YFP fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between.
Part was derived by cloning of BBa_K157002, BBa_K157009 and BBa_K157007.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN IEDGGVQLAD HYQQNTPIGD GPVLLPDNHY LSYQSKLSKD PNEKRDHMVL LEFVTAAGIT HGMDELYKTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGACG GCGGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CACGGCATGG ACGAGCTGTA CAAGACCGGT TAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157015 BBa_K157015]
Description:
N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
Part was derived by cloning of BBa_K157002, BBa_K157008.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGMV SKGEELFTGV VPILVELDGD VNGHKFSVSG EGEGDATYGK LTLKLICTTG KLPVPWPTLV TTLYLQCFAR YPDHMKQHDF FKSAMPEGYV QERTIFFKDD GNYKTRAEVK FEGDTLVNRI ELKGIDFKED GNILGHKLEY NYNSHNVYIT ADKQTG*
gene sequence:
ATGGCCGGCA TAGCTACCG GAATGGTGGG TGCACTTTTG CTCCTTTTGG TCGTTGCCCT GGGGATAGGA CTCTTTATGA CCGGCATGGT GTCCAAGGGC GAGGAACTGT TCACCGGCGT GGTGCCCATC CTGGTGGAGC TGGACGGCGA CGTGAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAA GGCGACGCCA CCTACGGCAA GCTGACCCTG AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTGGT GACCACCCTG TACCTCCAGT GCTTCGCCAG ATACCCCGAC CACATGAAGC AGCACGATTT CTTCAAGAGC GCCATGCCCG AGGGCTACGT GCAGGAACGG ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCAGAG CCGAAGTGAA GTTCGAGGGC GACACACTGG TGAACCGGAT CGAGCTGAAG GGCATCGACT TCAAAGAGGA CGGCAATATC CTGGGCCACA AGCTGGAATA CAACTACAAC AGCCACAACG TGTACATCAC CGCCGACAAG CAGACCGGTT AA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157016 BBa_K157016]
Description:
C-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between.
Part was derived by cloning of BBa_K157002, BBa_K157009 and BBa_K157005.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN
IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSKLSKD PNEKRDHMVL LEFVTAAGIT LGMDELYKTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGATG GCAGCGTGCA GCTGGCCGAT CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CTGGGCATGG ACGAGCTGTA CAAGACCGGT TAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157017 BBa_K157017]
Description:
N-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
Part was derived by cloning of BBa_K157002, BBa_K157006.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGMS KGEELFTGVV PILVELDGDV NGHKFSVSGE GEGDATYGKL TLKFICTTGK LPVPWPTLVT TLGVQCFARY PDHMKRHDFF KSAMPEGYVQ ERTIFFKDDG NYKTRAEVKF EGDTLVNRIE LKGIDFKEDG NILGHKLEYN AISDNVYITA
DKQTG*
gene sequence:
ATGGCCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTG
GGGATAGGACTCTTTATGACCGGCATGAGCAAGGGCGAGGAACTGTTCACCGGCGTGGTG
CCCATCCTGGTGGAGCTGGACGGCGACGTGAACGGCCACAAGTTCAGCGTGTCCGGCGAG
GGCGAAGGCGACGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAG
CTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGGGCGTGCAGTGCTTCGCCAGATAC
CCCGACCACATGAAGCGGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAG
GAACGGACCATCTTCTTCAAGGACGACGGCAACTACAAGACCAGAGCCGAAGTGAAGTTC
GAGGGCGACACACTGGTGAACCGGATCGAGCTGAAGGGCATCGACTTCAAAGAGGACGGC
AATATCCTGGGCCACAAGCTGGAATACAACGCCATCAGCGACAACGTGTACATCACCGCC
GACAAGCAGACCGGTTAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157018 BBa_K157018]
Description:
First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
Part was derived by cloning of the parts BBa_K157002, BBa_I757011.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVGALLLLLVVALGIGLFMTGHPETLAKVKDAEDQLGARVGYIELDLNSGKIL
ESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGM
TVGELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRWEPELNEAIPNDE
RDTTTPVAMATTLRKLLTGELLGTG*
gene sequence:
´ATGGCCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTG
GGGATAGGACTCTTTATGACCGGCCACCCGGAAACCCTGGCCAAAGTGAAAGATGCGGAA
GATCAGCTGGGTGCGCGTGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTG
GAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGC
GGTGCGGTTCTGAGCCGTATTGATGCGGGCCAGGAACAGCTGGGCCGTCGTATTCATTAT
AGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATG
ACCGTGGGCGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTG
CTGCTGACCACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCGTAACATGGGCGAT
CATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAA
CGTGATACCACCACCCCGGTGGCCATGGCGACCACCCTGCGTAAACTGCTGACCGGCGAA
CTGCTGGGCACCGGTTAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157019 BBa_K157019]
Description:
Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
Part was derived by cloning of BBa_K157002, BBa_I757012.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVGALLLLLVVALGIGLFMTGGTPASRQQLMDWMKADKVAGPLLRSVLPAGWF
IADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDELNRQIAEIGASLIKHWTG
gene sequence:
ATGGCCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTG
GGGATAGGACTCTTTATGACCGGCGGTACTCCGGCTTCCCGGCAACAATTAATGGACTGG
ATGAAAGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGTGCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACTGAATCGTCAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGACCGGT
TAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157020 BBa_K157020]
Description:
Part 1 of the Split- Luciferase(BB057) fused to the trans-membrane region of the Erbb1/EGF-receptor.
Part was derived by cloning of BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVGALLLLLVVALGIGLFMTGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCT
TGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDTG*
gene sequence:
ATGGCCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTG
GGGATAGGACTCTTTATGACCGGCGAAGCGATTGTGGATATTCCGGAAATTCCGGGCTTT
AAAGATCTGGAACCGATGGAACAGTTTATTGCGCAGGTGGATCTGTGCGTGGATTGCACC
ACCGGCTGCCTGAAAGGCCTGGCCAACGTGCAGTGCAGCGATCTGCTGAAAAAATGGCTG
CCGCAGCGTTGCGCGACCTTTGCGAGCAAAATTCAGGGCCAGGTGGATAAAATTAAAGGC
GCGGGTGGCGATACCGGTTAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157021 BBa_K157021]
Description:
Second part of Split-Luciferase(BB058) fused to the trans-membrane region of the Erbb1/EGF-receptor.
Part was derived by cloning of the parts BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVGALLLLLVVALGIGLFMTGKPTENNEDFNIVAVASNFATTDLDADRGKLPG
KKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIG
TG*
gene sequence:
ATGGCCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTG
GGGATAGGACTCTTTATGACCGGCAAACCGACCGAAAACAACGAAGATTTTAACATTGTG
GCGGTGGCGAGCAACTTTGCGACCACCGATCTGGATGCGGATCGTGGCAAACTGCCGGGC
AAAAAACTGCCGCTGGAAGTGCTGAAAGAAATGGAAGCGAACGCGCGTAAAGCCGGTTGC
ACCCGTGGCTGCCTGATTTGCCTGAGCCATATTAAATGCACCCCGAAAATGAAAAAATTT
ATCCCGGGTCGTTGCCATACCTATGAAGGCGATAAAGAAAGCGCGCAGGGCGGCATTGGC
ACCGGTTAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157022 BBa_K157022]
Description:
The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor.
Part was derived by cloning of BBa_K157001, BBa_K157003 and BBa_K157013.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGTTAA
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157023 BBa_K157023]
Description:
Lipocalin (with a flexible GGGS-linker at the C-terminus) fusioned to the signal peptide of Erbb1/EGF-receptor.
Part was derived by cloning of BBa_K157001, BBa_K157003 and BBa_K157013.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGTTAA
- SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157024 BBa_K157024]
Description:
A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase.
Part was derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTGIATGMVG
ALLLLLVVALGIGLFMTGHPETLAKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFP
MMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVGELCSAAI
TMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRWEPELNEAIPNDERDTTTPVAMA
TTLRKLLTGELLGTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCCACCCG
GAAACCCTGGCCAAAGTGAAAGATGCGGAAGATCAGCTGGGTGCGCGTGTGGGCTATATT
GAACTGGATCTGAACAGCGGCAAAATTCTGGAATCTTTTCGTCCGGAAGAACGTTTTCCG
ATGATGAGCACCTTTAAAGTGCTGCTGTGCGGTGCGGTTCTGAGCCGTATTGATGCGGGC
CAGGAACAGCTGGGCCGTCGTATTCATTATAGCCAGAACGATCTGGTGGAATATAGCCCG
GTGACCGAAAAACATCTGACCGATGGCATGACCGTGGGCGAACTGTGCAGCGCGGCGATT
ACCATGAGCGATAACACCGCGGCGAACCTGCTGCTGACCACCATTGGCGGTCCGAAAGAA
CTGACCGCGTTTCTGCGTAACATGGGCGATCATGTGACCCGTCTGGATCGTTGGGAACCG
GAACTGAACGAAGCGATTCCGAACGATGAACGTGATACCACCACCCCGGTGGCCATGGCG
ACCACCCTGCGTAAACTGCTGACCGGCGAACTGCTGGGCACCGGTTAA
- SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157025 BBa_K157025]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase.
Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757012.
For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVGALLLLLVVALGIGLFMTGKPTENNEDFNIVAVASNFATTDLDADRGKLPG
KKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIG
TG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCGGTACT
CCGGCTTCCCGGCAACAATTAATGGACTGGATGAAAGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGTGCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACTGAATCGTCAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGACCGGTTAA
- SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157026 BBa_K157026]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus.
Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTGIATGMVG
ALLLLLVVALGIGLFMTGRPACKIPNDLKQKVMNHTGKNGIKANFKIRHNIEDGGVQLAD
HYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKTG
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCCGACCA
GCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAAGAACGGC
ATCAAGGCCAACTTCAAGATCCGGCACAACATCGAGGACGGCGGCGTGCAGCTGGCCGAC
CACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTAC
CTGAGCTACCAGAGCAAGCTGTCCAAGGACCCCAACGAGAAGCGGGACCACATGGTGCTG
CTGGAATTTGTGACAGCCGCCGGAATCACCCACGGCATGGACGAGCTGTACAAGACCGGT
TAA
- SP-AntiNIP-GGGSlinker-Tm-nYFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157027 BBa_K157027]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP.
Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTGIATGMVG
ALLLLLVVALGIGLFMTGMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
LTLKLICTTGKLPVPWPTLVTTLYLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCATGGTG
TCCAAGGGCGAGGAACTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGACGGCGAC
GTGAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAAGGCGACGCCACCTACGGCAAG
CTGACCCTGAAGCTGATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTG
ACCACCCTGTACCTCCAGTGCTTCGCCAGATACCCCGACCACATGAAGCAGCACGATTTC
TTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAACGGACCATCTTCTTCAAGGACGAC
GGCAACTACAAGACCAGAGCCGAAGTGAAGTTCGAGGGCGACACACTGGTGAACCGGATC
GAGCTGAAGGGCATCGACTTCAAAGAGGACGGCAATATCCTGGGCCACAAGCTGGAATAC
AACTACAACAGCCACAACGTGTACATCACCGCCGACAAGCAGACCGGTTAA
- SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157028 BBa_K157028]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus.
Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTGIATGMVG
ALLLLLVVALGIGLFMTGRPACKIPNDLKQKVMNHTGKNGIKANFKIRHNIEDGSVQLAD
HYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKTG
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCCGACCA
GCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAAGAACGGC
ATCAAGGCCAACTTCAAGATCCGGCACAACATCGAGGATGGCAGCGTGCAGCTGGCCGAT
CACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTAC
CTGAGCACCCAGAGCAAGCTGTCCAAGGACCCCAACGAGAAGCGGGACCACATGGTGCTG
CTGGAATTTGTGACAGCCGCCGGAATCACCCTGGGCATGGACGAGCTGTACAAGACCGGT
TAA
- SP-AntiNIP-GGGSlinker-Tm-nCFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157029 BBa_K157029]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and intracellular the N-terminal part of the split fluorophor YFP. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTGIATGMVG
ALLLLLVVALGIGLFMTGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
TLKFICTTGKLPVPWPTLVTTLGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCATGAGC
AAGGGCGAGGAACTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGACGGCGACGTG
AACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAAGGCGACGCCACCTACGGCAAGCTG
ACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACC
ACCCTGGGCGTGCAGTGCTTCGCCAGATACCCCGACCACATGAAGCGGCACGATTTCTTC
AAGAGCGCCATGCCCGAGGGCTACGTGCAGGAACGGACCATCTTCTTCAAGGACGACGGC
AACTACAAGACCAGAGCCGAAGTGAAGTTCGAGGGCGACACACTGGTGAACCGGATCGAG
CTGAAGGGCATCGACTTCAAAGAGGACGGCAATATCCTGGGCCACAAGCTGGAATACAAC
GCCATCAGCGACAACGTGTACATCACCGCCGACAAGCAGACCGGTTAA
- SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157030 BBa_K157030]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase(BB058).
Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTGIATGMVG
ALLLLLVVALGIGLFMTGKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKE
MEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCAAACCG
ACCGAAAACAACGAAGATTTTAACATTGTGGCGGTGGCGAGCAACTTTGCGACCACCGAT
CTGGATGCGGATCGTGGCAAACTGCCGGGCAAAAAACTGCCGCTGGAAGTGCTGAAAGAA
ATGGAAGCGAACGCGCGTAAAGCCGGTTGCACCCGTGGCTGCCTGATTTGCCTGAGCCAT
ATTAAATGCACCCCGAAAATGAAAAAATTTATCCCGGGTCGTTGCCATACCTATGAAGGC
GATAAAGAAAGCGCGCAGGGCGGCATTGGCACCGGTTAA
- SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157031 BBa_K157031]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase(BB058).
Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGQVQLQQPGAELVKPGASVKLSCKASGYTFTS
YWMHWVKQRPGRGLEWIGRIDPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDS
AVYYCARYDYYGGSYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSQAVVTQESALTTSPGE
TVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALT
ITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGTGGGGGSGGGGSGGGGSTGIATGMVG
ALLLLLVVALGIGLFMTGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANV
QCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCCAGGTGCAGCTCCAGCAGCCCGGAGCCGAACTG
GTGAAGCCAGGCGCCAGCGTGAAGCTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGC
TACTGGATGCACTGGGTGAAACAGAGGCCCGGCAGAGGCCTGGAATGGATCGGCCGGATC
GACCCCAACAGCGGCGGCACCAAGTACAACGAGAAGTTCAAGAGCAAGGCCACCCTGACC
GTGGACAAGCCCAGCAGCACCGCCTACATGCAGCTGTCCAGCCTGACCAGCGAGGACAGC
GCCGTGTACTACTGCGCCAGATACGACTACTACGGCGGCAGCTACTTCGACTACTGGGGC
CAGGGCACCACCGTGACCGTGTCCTCTGGGGGAGGGGGCTCAGGAGGAGGAGGAAGCGGG
GGAGGGGGCAGCCAGGCCGTGGTGACCCAGGAAAGCGCCCTGACCACCTCCCCTGGCGAG
ACAGTGACCCTGACCTGCCGGTCCTCTACAGGCGCCGTGACCACAAGCAACTACGCCAAC
TGGGTGCAGGAAAAGCCCGACCACCTGTTCACCGGCCTGATCGGCGGCACAAACAACAGA
GCCCCTGGCGTGCCCGCTAGATTCAGCGGCAGCCTGATCGGGGACAAGGCCGCCCTGACA
ATCACAGGCGCCCAGACCGAGGACGAGGCCATCTACTTTTGCGCCCTGTGGTACAGCAAC
CACTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCACCGGCGGAGGAGGTGGT
TCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCCACCGGCATAGCTACCGGAATGGTGGGT
GCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGGATAGGACTCTTTATGACCGGCGAAGCG
ATTGTGGATATTCCGGAAATTCCGGGCTTTAAAGATCTGGAACCGATGGAACAGTTTATT
GCGCAGGTGGATCTGTGCGTGGATTGCACCACCGGCTGCCTGAAAGGCCTGGCCAACGTG
CAGTGCAGCGATCTGCTGAAAAAATGGCTGCCGCAGCGTTGCGCGACCTTTGCGAGCAAA
ATTCAGGGCCAGGTGGATAAAATTAAAGGCGCGGGTGGCGATACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157032 BBa_K157032]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker , the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split enzyme beta-Lactamase.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757011.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGHPETLAKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCG
AVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVGELCSAAITMSDNTAANLL
LTTIGGPKELTAFLRNMGDHVTRLDRWEPELNEAIPNDERDTTTPVAMATTLRKLLTGEL
LGTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCCACCCGGAAACCCTGGCCAAAGTGAAAGATGCGGAAGAT
CAGCTGGGTGCGCGTGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTGGAA
TCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGCGGT
GCGGTTCTGAGCCGTATTGATGCGGGCCAGGAACAGCTGGGCCGTCGTATTCATTATAGC
CAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATGACC
GTGGGCGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTGCTG
CTGACCACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCGTAACATGGGCGATCAT
GTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAACGT
GATACCACCACCCCGGTGGCCATGGCGACCACCCTGCGTAAACTGCTGACCGGCGAACTG
CTGGGCACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157033 BBa_K157033]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the transmembrane region of the Erbb1/EGF-receptor and the C-terminal part of the split enzyme beta-Lactamase.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757012.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGGTPASRQQLMDWMKADKVAGPLLRSVLPAGWFIADKSGAGERGSRGIIAALGP
DGKPSRIVVIYTTGSQATMDELNRQIAEIGASLIKHWTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCGGTACTCCGGCTTCCCGGCAACAATTAATGGACTGGATG
AAAGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGTGCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACTGAATCGTCAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157034 BBa_K157034]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGRPACKIPNDLKQKVMNHTGKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDG
PVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
GTTATGAATCACACCGGCAAGAACGGCATCAAGGCCAACTTCAAGATCCGGCACAACATC
GAGGACGGCGGCGTGCAGCTGGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGC
CCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGAGCAAGCTGTCCAAGGACCCC
AACGAGAAGCGGGACCACATGGTGCTGCTGGAATTTGTGACAGCCGCCGGAATCACCCAC
GGCATGGACGAGCTGTACAAGACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-nYFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157035 BBa_K157035]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP. Part was derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGK
LPVPWPTLVTTLYLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKF
EGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCATGGTGTCCAAGGGCGAGGAACTGTTCACCGGCGTGGTG
CCCATCCTGGTGGAGCTGGACGGCGACGTGAACGGCCACAAGTTCAGCGTGTCCGGCGAG
GGCGAAGGCGACGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCACCGGCAAG
CTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGTACCTCCAGTGCTTCGCCAGATAC
CCCGACCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAG
GAACGGACCATCTTCTTCAAGGACGACGGCAACTACAAGACCAGAGCCGAAGTGAAGTTC
GAGGGCGACACACTGGTGAACCGGATCGAGCTGAAGGGCATCGACTTCAAAGAGGACGGC
AATATCCTGGGCCACAAGCTGGAATACAACTACAACAGCCACAACGTGTACATCACCGCC
GACAAGCAGACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157036 BBa_K157036]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor CFP with a fluolinker joined to its N-Terminus.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGRPACKIPNDLKQKVMNHTGKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDG
PVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
GTTATGAATCACACCGGCAAGAACGGCATCAAGGCCAACTTCAAGATCCGGCACAACATC
GAGGATGGCAGCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGC
CCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGAGCAAGCTGTCCAAGGACCCC
AACGAGAAGCGGGACCACATGGTGCTGCTGGAATTTGTGACAGCCGCCGGAATCACCCTG
GGCATGGACGAGCTGTACAAGACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-nCFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157037 BBa_K157037]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor CFP.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157006.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKL
PVPWPTLVTTLGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
GDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCATGAGCAAGGGCGAGGAACTGTTCACCGGCGTGGTGCCC
ATCCTGGTGGAGCTGGACGGCGACGTGAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGC
GAAGGCGACGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTG
CCCGTGCCCTGGCCCACCCTGGTGACCACCCTGGGCGTGCAGTGCTTCGCCAGATACCCC
GACCACATGAAGCGGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAA
CGGACCATCTTCTTCAAGGACGACGGCAACTACAAGACCAGAGCCGAAGTGAAGTTCGAG
GGCGACACACTGGTGAACCGGATCGAGCTGAAGGGCATCGACTTCAAAGAGGACGGCAAT
ATCCTGGGCCACAAGCTGGAATACAACGCCATCAGCGACAACGTGTACATCACCGCCGAC
AAGCAGACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157038 BBa_K157038]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLP
QRCATFASKIQGQVDKIKGAGGDTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCGAAGCGATTGTGGATATTCCGGAAATTCCGGGCTTTAAA
GATCTGGAACCGATGGAACAGTTTATTGCGCAGGTGGATCTGTGCGTGGATTGCACCACC
GGCTGCCTGAAAGGCCTGGCCAACGTGCAGTGCAGCGATCTGCTGAAAAAATGGCTGCCG
CAGCGTTGCGCGACCTTTGCGAGCAAAATTCAGGGCCAGGTGGATAAAATTAAAGGCGCG
GGTGGCGATACCGGTTAA
- SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157039 BBa_K157039]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of Splitluciferase.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg.
For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAGAALLALLAALCPASRATGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAK
YPSPNGKYGKCGWAEYTPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVG
GYTRKTVFNVLSTDNKNYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIG
SPVVDSQKLVYSDFSEAACKVNNTGGGGGSGGGGSGGGGSTGIATGMVGALLLLLVVALG
IGLFMTGKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCT
RGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGTG*
gene sequence:
ATGGCCGGCATGAGACCATCTGGTACTGCTGGAGCCGCATTGCTGGCACTTTTGGCTGCG
CTGTGCCCTGCAAGCAGAGCAACCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTG
AAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCAAG
TACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGC
AAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGC
ACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATC
GGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGCTG
TCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGC
AGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAA
GTGAACAACACCGGCGGAGGAGGTGGTTCAGGTGGTGGTGGTAGTGGAGGAGGAGGATCC
ACCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTGGGG
ATAGGACTCTTTATGACCGGCAAACCGACCGAAAACAACGAAGATTTTAACATTGTGGCG
GTGGCGAGCAACTTTGCGACCACCGATCTGGATGCGGATCGTGGCAAACTGCCGGGCAAA
AAACTGCCGCTGGAAGTGCTGAAAGAAATGGAAGCGAACGCGCGTAAAGCCGGTTGCACC
CGTGGCTGCCTGATTTGCCTGAGCCATATTAAATGCACCCCGAAAATGAAAAAATTTATC
CCGGGTCGTTGCCATACCTATGAAGGCGATAAAGAAAGCGCGCAGGGCGGCATTGGCACC
GGTTAA
- Transfection_Vector-(BBa_J52017)-CMV
Part Name:
[http://partsregistry.org/Part:BBa_K157040 BBa_K157040]
Description:
Transfection vector with CMV Promotor for expression in eukaryotic cells.
gene sequence:
see [http://partsregistry.org/cgi/partsdb/puttext.cgi BBa_K157040_gene_sequence]
- Transfection_Vector-(BBa_J52017)-CMV-CFP
Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157041 BBa_K157041]
Description:
Transfectionvector with CMV promoter and CFP as reporter.
gene sequence:
see [http://partsregistry.org/cgi/partsdb/puttext.cgi BBa_K157041_gene_sequence]
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