Team:Hawaii/Notebook/2008-07-11

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Things we did today

Wetlab work

Plasmid prep of Biobricks

Krystle
  • Plasmid prep for B0015, E0026, R0010, J33207, B0030

Construction of pnir, slr2016, pilA with vector

Grace
  • Extracted DNA from gel using Qiagen MiniElute Gel Extraction Kit
  • Ligated pnir, slr2016, pilA with BBa_C0012 harboring vector
  • Ligated C0012 with vector as positive control
  • Ligated annealed products with vector
  • Ran ligations on gel
  • Annealed products + vector = smear
  • Rest of gel blank = not enough DNA

Made stock solutions

Grace
  • For plasmid prep:
  • GTE
  • NaOH/SDS
  • Potassium Acetate
  • General:
  • 0.25 M D-glucose
  • Autoclaved nanopure water

Made DB3.1 competent cells

Margaret


Discussion

Quote of the Day

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]