Team:BCCS-Bristol/Calendar-Notebook/21 July 2008

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OD measurement

The OD of both strains was measured over a day so that we would know whether the bacteria were in their log phase in any experiments requiring innoculation of bacteria.

A, B and C show 100, 500 and 200µl innoculations respectively.


Time (minutes) OD600 (1.5 diluted)


MG1655MC1000
ABCABC
00.00160.00420.0170.00160.00440.0174
600.0080.0130.0730.0060.0170.068
800.0110.0520.1090.0090.0460.123
1000.0190.0590.1420.0170.0670.167
1200.0290.0760.1690.0240.0830.183
1400.0390.0950.2130.0390.1440.226
1600.0630.1360.260.0530.1470.275
1800.0740.1660.2760.0690.1860.298
2000.1050.2070.3010.0890.220.316
2200.1340.250.3550.1160.2680.331
2400.1780.2760.3860.1510.2930.341
2600.2330.3290.4190.1980.3250.372
2800.2390.3340.4350.2030.3260.375
3000.2630.3630.4730.2380.340.388
3200.2960.3910.4910.270.3640.412
3400.3190.4250.5110.290.3780.429
3600.320.4350.5120.2940.3820.432
3800.3920.5420.5140.3110.4340.438
4000.4350.5490.5880.3480.4570.497
4200.470.520.4870.3820.4620.504
4400.4830.5860.6330.4770.4370.516
4600.4980.4820.6290.4110.4840.517
4800.5740.6430.7210.4510.5160.524
5000.6070.660.730.4550.5140.515
5200.650.6730.7230.4530.5220.523

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BCCS Bristol-wetlab-OD measurments.JPG

Chemotaxis experiment for MG1655 showed no real difference btween the control (water) and the aspartate. may have been due to higher agar concentration caused by an unusually large water loss in autoclaving, causing high concentrations of agar.

Started on the transformation of bacteria with biobrick from paper, punched out hole and put in TE buffer (below) following protocol on igem sheet.

Chemotaxis experiment with 20% aspartate repeated.

DMSO stocks of master cultures made as back up in the -80 o C freezer.