Team:Hawaii/Notebook/2008-09-25

From 2008.igem.org

Revision as of 21:56, 25 September 2008 by MargaretRuzicka (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of Ligation

Margaret
  • The plac/rbs+rep and oriTr(BB) transformations resulted in 0 colonies with the correct insert, so the remainder of the ligation reaction from 9/16 was digested with XbaI and run on a gel.

Sequencing & Colony PCR

Margaret
  • PCR
  • oriV colonies (3,4,6,8,9,10,11)
  • aadA(BB) colonies (4,5,7,8)
  • aadA(pRL) colonies (1,2)
  • oriT + J33207 (1,2,4)
  • oriT + J33207(de-phosphorylated) colony 1
  • 5 additional colonies of oriTr transformation were PCR'ed

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]