Team:Hawaii/Notebook/2008-09-28

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Things we did today

Wetlab work

Ligation

Margaret
  • Ligation of promoter/rbs to rep into pSB1A3 (dephosphorylated)
  • Ligation of pSB1A3 (dephosphorylated) to self

Transformation into DH5-alpha

Margaret
  • promoter/rbs to rep into pSB1A3 (dephosphorylated)
  • pSB1A3 (dephosphorylated) to self ligation
  • (+) pUC18
  • (-) no plasmid

Culture DH5-alpha with plamids

Margaret
  • aadA (BB)with promoter/rbs/terminator
  • aadA (pRL)with promoter/rbs/terminator
  • rep
  • oriV
  • oriT (J33207)
  • oriTr (J33207)


Drylab Work

Name of Task

Margaret
  • The sequence of rep (submitted 9/24) was verified using Seqman software
  • region between 1550-1600 was not covered by primers, so an additional primer was designed and ordered. Will submit again for sequencing


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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