Team:Chiba/Calendar-Home/8 September 2008

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7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract


--->Zymo Clean
->15μL?


Team:Output

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
Sample No.
DNA(digested sample) 76
SAP/td> 22
SAP Buffer 33
TOTAL 30μl30μl

-->37℃ 1hour

-->65℃ 30min

-->DNA purification(①:21μl,⑤:6μl)


-->Gel check

Sample No. each
DNA(digested sample) 1
Loading Dye 1
dH2O 4
TOTAL 6μl
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No. ①&②①&③①&④⑤&⑥
DNA(vector) 333352.1
DNA(insert) 333-3.1-
Ligase 111111
Ligase Buffer(x5) 222222
dH2O 11148.94
TOTAL 10μl10μl10μl10μl20μl10μl