Team:Heidelberg/Notebook/Sensing Group/Notebook/4thweek

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Contents

Monday, 08/25/2008

  • sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI

--> not successful

  • preparation of O/N culture of pDK48 transformed cells

Tuesday, 08/26/2008

Cloning

Digestion of pDK48 with xbaI and PstI yield expected results
  • digestion of pDK48 with XbaI and PstI to check wether the vector is the right one
  • digestion of Fusion-1 (from 08/21/2008) with NcoI/NdeI, with subsequent gelextraction
  • Ligation of Fusion-1 and pDk48 and transformation into DH5a competent cells

LuxS Test


Wednesday, 08/27/2008

Thursday, 08/28/2008

Friday, 08/29/2008

Go to 5th week